The second qualifying round features four new teams aiming for a spot in the finals. Along with the other obstacles, Bungee Road is a new fifth roadblock on the course. Golden Hearts feature Neil Craver, Grant McCartney, and Natalie Duran. NorCal Ninjas include David Campbell, Sean Noble, and Rachel Mulvaney. Towers of Power consists of Brandon Mears, Dan Polizzi, and Selena Laniel. Team Midoryama features Ian Dory, Meagan Martin, and Dan Yager.