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Yes

Shin'ichirou Miki as Elijah Vashlov (voice)
Tom Holland as Japanese Beetle (voice)
Stephen Tobolowsky as Tabaqui
Koki Uchiyama as Velvet
Kohei Amasaki as Kiriyama
Frank Welker as Mad Dog
Joanna Lumley as Annie (voice)
Maitreyi Ramakrishnan as Zipp Storm
Oliver Ford Davies as Sir Alexander Cadogan / Anthony Eden / Owen O'Malley (voice)
Yui Ishikawa as 物月珠美 / Jumi Monozuki
Hiroshi Kamiya as Kakushi Gotou (voice)
Nobuo Tobita as 川路利良 / Toshiyoshi Kawaji
James Arnold Taylor as Ratchet (voice)
Emily Carey as Sh’eenaz
Daisuke Hirakawa as Izo Masubuchi
Laney Shur as Gwen Stacy / Ghost-Spider
Daryl Sabara as Marco North (voice)
Chris Coppola as Comet (voice)
Aysin Isimer as Sevim
Lex Lang as Incidental Characters (voice)
Katherine Sun as Eggy
Ryan McPartlin as Clutch Powers (voice)
Dorothée Pousséo as Shamira

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