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Yes

Laney Shur as Gwen Stacy / Ghost-Spider
Jack Mercer as Popeye / Pappy (voice)
Glutenhead as Him (voice)
Kelly Stables as Athena / Puffy (voice)
Takahiro Sakurai as Giyu Tomioka
Nobuo Tobita as 川路利良 / Toshiyoshi Kawaji
John Boyle as (voice)
Luke Youngblood as Mitch
Alan Cumming as Gutsy Smurf (voice)
Jurota Kosugi as Tooyama (voice)
Eiko Masuyama as Fujiko Mine (voice)
Shannon Settlemyre as Hello Kitty
Misato Fukuen as Secchan (voice)
Xander Mobus as Romani Archaman (voice)
Tamao Satô as Narrator (voice)
John Slattery as Arthur (voice)
Ron Glass as Dr. Lazenby
Adrian Holmes as Dylan
Sid Raymond as Katnip
Callum Scott Howells as Berry
Saori Hayami as Kotoha Hanami / Cure Felice
Traolach O'Riordain as Doughboy (voice)
Cam Clarke as Aladdin (voice)
Kj Schrock as Frog / Additional Voices (voice)

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